recombinant enzymes meaning in Chinese

重组酶

According to the purity and the activity recovery , the recombinant enzyme was well purified by both of these two separation combinations . like natural gloshedobin , the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate , but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly
与天然蛇毒类凝血酶一致，当用三肽p山itroanilide衍生物作为底物时，分泌表达的重组大连蛇岛蝗蛇毒类凝血酶具有较强的生色底物活性，但用精氨酸甲酯如tame （ na厂tosyl l ar 。
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
将大连蛇岛蝮蛇和长白山白眉蝮蛇毒类凝血酶基因分别克隆到大肠杆菌表达载体pet - 32 ( a ) +中，在t7启动子下表达出融合蛋白，融合部分为硫氧还蛋白，位于类凝血酶基因上游，并在其n端带6xhistag标签以利于表达产物的分离纯化，经热激转化至宿主菌bl21 ( de3 )中， iptg诱导斗小时后收获菌体。